primary rabbit polyclonal antibodies against ki 67  (Cell Signaling Technology Inc)

Journal: Experimental eye research

Article Title: Effects of MMP12 on cell motility and inflammation during corneal epithelial repair

doi: 10.1016/j.exer.2017.04.007

Figure Lengend Snippet: (A) Immunofluorescence of cell adhesion markers Zo-1 and E-cadherin in wounded primary epithelial cell cultures from WT and Mmp12-/- corneas. Images are of cells at the wound edge or peripheral to the wound edge. Scale bars: 50 μm. (B) Micrographs of representative whole-mount preparations of central corneas stained for the proliferation marker Ki-67 in unwounded and wounded (24 hours after injury) corneas. Scale bars: 100 μm. Dapi staining of cell nuclei was used to assess wound closure. (C) Quantification of Ki-67 cell counts in the central corneas of wounded WT and Mmp12-/- mice 24 hours after injury. Mean ± s.e.m. Ki-67 + cell counts are shown and are 823 (WT, n=8) and 770 (Mmp12-/-; n= 16).

Article Snippet: Immunostaining was performed using a rabbit polyclonal primary antibody against Ki-67 (Fisher Scientific, Hampton, NH; #PIPA519462), and a rat primary monoclonal antibody against Gr-1 (BD Pharmingen, San Jose, CA; #550291) overnight at 4°C, followed by overnight incubation at 4°C with secondary antibodies Alexa 488-conjungated goat anti-rabbit and Alexa 488-conjungated goat anti-rat (Invitrogen, Carlsbad, CA).

Techniques: Immunofluorescence, Staining, Marker